Rat model of hepatic warm ischemia reperfusion injury with obstructive jaundice

Abstract

Objective To establish the model of hepatic warm ischemia reperfusion injury with obstructive jaundice on sprague-dawley rats. Methods One hundred and two mature SPF sprague-dawley rats were used in the establishment. Among them 40 rats were randomly divided into 4 groups: sham group, 7 days obstructive jaundice group, 14 days obstructive jaundice group and 21 days obstructive jaundice group, with 10 rats in each group. Double ligation and cutting the common bile ducts in the 7, 14, and 21 d groups were used to investigate the optimal time of obstruction. The other 62 rats were randomly divided into 4 groups after 7 days of obstruction: non-ischemia group (0 min group, n=10), 15 min ischemia group (n=12), 30 min ischemia group (n=16) and 45 min ischemia group (n=24). Pringle method of blocking hepatic portal vessels was used for 0, 15, 30 and 45 min correspondingly. Liver function, survival rate and pathological changes were observed respectively. Results The model of hepatic warm ischemia reperfusion injury was better established after obstruction for 7 days on sprague-dawley rat. After 7 days of obstructive jaundice, along with the prolongation of Pringle method of blocking hepatic portal vessels, the liver function decreased progressively and the mortality rate increased gradually. There were statistical differences between the 4 groups (P<0.05). The hepatic pathological changes such as the degeneration and necrosis of hepatic cells were more severe gradually with the ischemia time going. The mortality rate was 50% after 30 min hepatic portal vessels blocking on 7 days obstructive jaundice rats, and there was serious degeneration and necrosis of hepatic cells after 2 hours of reperfusion. Conclusion Obstruction for 7 days on sprague-dawley rats and then blocking hepatic portal vessels for thirty minutes can effectively establish an appropriate model of obstructive jaundice and hepatic warm ischemia reperfusion injury rats. Key words: Jaundice, obstructive; Reperfusion injury; Ischemia; Rats