Abstract
This paper describes the use of a radio-immunoassay system to determine the amount of free and total insulin in the plasma of diabetic subjects with varying levels of antibodies to insulin. The binding of 1 2 5I-labelled insulin by diabetic plasma is used as a measure of antibody present. After estimation of total insulin values standardization of this antibody binding has been made, using commercially available anti-serum to insulin. Standard conditions for taking the sample are not required, as antibody titre values estimated by this method are not influenced by the time of day or the dose of insulin administered to the patient. This also enables antibody levels to be compared, over a period of time, to allow longitudinal study of the clinical diabetic state and response to insulin therapy.
Published Version
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