Abstract
Simple SummaryFish oil is rich in long-chain n-3 polyunsaturated fatty acids (n-3 LC-PUFA), which play an important role in the regulation of oxidative stress and inflammatory response. In the present study, increasing n-3 LC-PUFA in the maternal diet with fish oil from the 84th day of gestation until the 16th day of lactation decreased the oxidative stress and inflammatory response in sows and enhanced the parameters related to the antioxidative capacity. However, the inflammatory response of suckling piglet increased pre-/post-lipopolysaccharide (LPS) challenge. We concluded that the maternal diet with fish oil might decrease the oxidative stress and inflammatory response in sows, and enhance the antioxidative ability but increase the susceptibility to inflammatory stimulation in their progenies.The aim of this study is to investigate the effect of the maternal diet with fish oil on the oxidative stress and inflammatory response in sows, and the protective effect on the piglets suckling the sows fed the diet with fish oil in the context of inflammatory stimulation. Twelve sows were divided into two groups. Sows were fed soybean oil diet (SD) or soybean oil + fish oil diet (FD) from gestation to lactation period. The blood samples of sows were collected from the auricular vein at the 16th day of lactation. One piglet was selected from each litter on the 14th day after birth. Lipopolysaccharide (LPS) was injected into the neck muscle after pre-treatment blood samples were collected from the anterior vena cava of piglets. The blood samples of piglets were collected at 5 h and 48 h post-LPS injection from the front cavity vein. Liver samples were collected at 48 h post-LPS injection. The FD diet significantly increased the level of high-density lipoprotein cholesterol (HDL-C) in the plasma of lactating sow, decreased the levels of alkaline phosphatase(AKP) and tumor necrosis factor alpha(TNF-α) in the plasma of lactating sows, and increased the level of immunoglobulin G(IgG) in the colostrum and interleukin-10(IL-10) in the milk (p < 0.05). In the FD group, the levels of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) significantly increased in the plasma of piglets at 48 h post-LPS injection (p < 0.05). Meanwhile, the relative expression of GSH-Px mRNA was decreased in the FD group (p < 0.05). However, the levels of interleukin-1 beta (IL-1β) and interleukin-6(IL-6) in the plasma of piglets were significantly higher in the FD group pre- and post-LPS injection (p < 0.05). The ratio of the phosphonated extracellular regulated protein kinases to the extracellular regulated protein kinases (p-ERK/ERK) protein in the livers of piglets was decreased (p < 0.05), but the expression of nuclear transcription factor-κB (NF-κB) mRNA and the ratio of the phosphonated inhibitor of NF-κB to the inhibitor of NF-κB (p-IκB-α/IκB-α) protein was increased in the livers of piglets (p < 0.05). These results indicate that a maternal diet with fish oil might decrease the oxidative stress and inflammatory response in sows, and enhance the antioxidative ability but increase the susceptibility to inflammatory stimulation in their progenies.
Highlights
Neonatal piglet survival is the major problem in the modern pig industry, because the imperfect immune system of neonatal piglet makes it sensitive to the pathogen
There were no differences in the relative intestinal length of piglets between the soybean oil diet (SD) group and the fish oil diet (FD) group
We show that the maternal diet with fish oil might reduce the inflammatory response and improve the liver function of lactating sows, our previous study showed that
Summary
Neonatal piglet survival is the major problem in the modern pig industry, because the imperfect immune system of neonatal piglet makes it sensitive to the pathogen. The lactating diet of a sow with 3–5% fish oil is beneficial for the health of suckling piglet by changing the fatty acid composition, increasing the immunoglobulins and/or decreasing the level of cytokines in the milk [7,8,9]. The effect of the maternal diet with fish oil on the immunomodulation of the progeny in the context of LPS-induced inflammation is unclear. The maternal diet with fish oil prolonged the gestational duration, alleviated oxidative stress in sows on the farrowing day and modulated inflammatory response in sows and their offspring [10]. We hypothesized that the maternal diet with fish oil could change the inflammatory response of the sows and potentiate the resistance to inflammation in the suckling piglets under the stimulation of immune stress
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