Abstract
The objectives of this chapter are to cover the current status of and future prospects for combined high-performance liquid chromatography/mass spectrometry (HPLC/MS) with particular emphasis on its ability to assist in the analysis of organic compounds relevant to endocrinological research. Initially, the currently most useful methods for HPLC/MS and techniques which hold promise for future development will be discussed. This will be followed by a description of areas relevant to endocrinology where HPLC/MS has been successfully applied. The off-line combination of HPLC with mass spectrometry, whereby peaks of interest are collected from the liquid chromatograph and are then transferred to the mass spectrometer, has been extensively used (Desiderio and Fridland), but will not be covered in this review.
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