Abstract
Molecularly imprinted polymers (MIP) are synthetic receptors that have an ability to recognize and select its template molecule from complex matrix. Nowadays many new approaches of MIPs are researched. One of them is the preparing of magnetic MIPs. Magnetic molecularly imprinted polymer (MMIP) nanoparticles can be a candidate for solid phase extraction adsorbent by the porous morphology, narrow size distribution, stable chemical and thermal property. The aim of this study is to prepare MMIP nanoparticles for solid phase extraction of fructose from apple juice. The PEG treated Fe3O4 magnetic nanoparticles surface was coated with monosaccharide (fructose) imprinted polymer. The monomer acrylamide, cross-linking agent ethylene glycol dimethacrylate (EGDMA), initiator azobisisobutyronitrile (AIBN), the porogen dimethylsulfoxide were used for fructose imprinting. The structural characterization of MMIP was performed by FT-IR, and the imprinting characteristics of polymer were also studied by adsorption experiment and Scatchard analysis. The 65% of fructose was recognized with fructose MMIP from apple juice using only an external permanent magnet without filtration/centrifugation.
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