Abstract
This chapter describes the location of cytochromes in Escherichia coli. In a study discussed in the chapter, Escherichia coli, strain B, was grown, harvested, and the cell free extracts were made by grinding with alumina powder, and extracting with 0.005 M 2-amino-2-hydroxymethylpropane-1:3-diol (tris) buffer pH 7.3 containing 0.01 M magnesium acetate. There is no evidence in bacterial cells for respiratory granules homogeneous in size and shape: the cytochromes are usually attached to granules of widely different sizes in the extracts and these granules probably derive from the breakdown of a larger structure, the cell membrane. Under some conditions, all the cytochrome system is bound to ghosts or cell membrane preparations, however, probably containing still some wall material. The ribonucleoprotein particles, which are uniform in size and seem to fill the bacterial cytoplasm, do not bear any cytochrome pigments. In E. coli extracts, granules bearing cytochromes can be separated from ribonucleoprotein particles. Moreover, the ghosts lysed in the presence of 0.01 M magnesium ions contain all the cytochromes of the cell.
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