Abstract
Ultraviolet microspectrophotometry of unfixed cells and freeze-dried cells of Vicia faba root tips has indicated that strong UV absorption in freezedried preparations of V. faba is caused by material in the cytoplasm which corresponds in appearance to granules previously described and claimed to contain DNA. Considerations of the properties of this substance indicates that it is not DNA. Meristematic nuclei of fresh and freeze-dried cells may absorb less UV than the surrounding cytoplasm, and photographs of such preparations may give the impression that the nuclei show anomalously weak absorptivity. However, quantitative photometry of such nuclei shows that the UV absorptivity of these nuclei is sufficient to account for the total amount of DNA these cells are known to contain. In spite of repeated attempts, we have not been able to find evidence suggesting that substantial amounts of DNA are anywhere in the cell outside of the nucleus.
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