Abstract
The lipids from rumen holotrich protozoa were isolated and partially identified. The lipid consisted of 70% phospholipids and 30% non-phospholipids. The phospholipids contained phosphatidyl ethanolamine (21%), phosphatidyl ethanolamine plasmalogen (22%), phosphatidyl choline (28%), and unknown phospholipids (29%). All the phospholipid fractions contained significant amounts of branched chain and unsaturated fatty acids. Degradation of the phosphatidyl ethanolamine and phosphatidyl choline with phospholipase A revealed that the branched chain and unsaturated acids were located in the beta position. Chemical degradation of the phosphatidyl ethanolamine plasmalogen indicated that the vinyl ether linkage was in the alpha position. The non-phospholipids consisted of a mixture of waxes, hydrocarbons, aliphatic alcohols, diglycerides, monoglycerides, hydroxyacids, unesterified fatty acids and sterols. The sterols and unesterified fatty acids comprised 50% of the fraction. The low concentration of stearic acid in the unesterified fatty acids (8.3%) raises a question as to the quantitative importance of holotrich protozoa in rumen hydrogenation.
Published Version
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