The level and distribution of the GABABR1 and GABABR2 receptor subunits in the rat's inferior colliculus

Lena Jamal,Chirag R Patel,Sehrish Butt,Aziz N Khan,Huiming Zhang

doi:10.3389/fncir.2012.00092

Abstract

The type B γ-aminobutyric acid receptor (GABAB receptor) is an important neurotransmitter receptor in the midbrain auditory structure, the inferior colliculus (IC). A functional GABAB receptor is a heterodimer consisting of two subunits, GABABR1 and GABABR2. Western blotting and immunohistochemical experiments were conducted to examine the expression of the two subunits over the IC including its central nucleus, dorsal cortex, and external cortex (ICc, ICd, and ICx). Results revealed that the two subunits existed in both cell bodies and the neuropil throughout the IC. The two subunits had similar regional distributions over the IC. The combined level of cell body and neuropil labeling was higher in the ICd than the other two subdivisions. Labeling in the ICc and ICx was stronger in the dorsal than the ventral regions. In spite of regional differences, no defined boundaries were formed between different areas. For both subunits, the regional distribution of immunoreactivity in the neuropil was parallel to that of combined immunoreactivity in the neuropil and cell bodies. The density of labeled cell bodies tended to be higher but sizes of cell bodies tended to be smaller in the ICd than in the other subdivisions. No systematic regional changes were found in the level of cell body immunoreactivity, except that GABABR2-immunoreactive cell bodies in the ICd had slightly higher optic density (OD) than in other regions. Elongated cell bodies existed throughout the IC. Many labeled cell bodies along the outline of the IC were oriented in parallel to the outline. No strong tendency of orientation was found in labeled cell bodies in ICc. Regional distributions of the subunits in ICc correlated well with inputs to this subdivision. Our finding regarding the contrast in the level of neuropil immunoreactivity among different subdivisions is consistent with the fact that the GABAB receptor has different pre- and postsynaptic functions in different IC regions.

Highlights

The type B γ-aminobutyric acid receptor (GABAB receptor) is an important neurotransmitter receptor in the midbrain auditory structure, the inferior colliculus (IC)
Only the GABABR2 subunit was probed. Both the GABABR1 and GABABR2 subunits were expressed at a higher level in the ICd than in the other two collicular subdivisions. This area difference was confirmed by all the other cases examined in this study (2 and 3 cases for the GABABR1 and GABABR2 subunits, respectively)
In an effort to minimize this effect, all photomicroscopic images were taken when the focal plane was at a depth of 10 μm below the tissue surface

Summary

Introduction

The type B γ-aminobutyric acid receptor (GABAB receptor) is an important neurotransmitter receptor in the midbrain auditory structure, the inferior colliculus (IC). Over the area of the IC in a section, a mean OD value was obtained for each of the alternating grid boxes to reflect www.frontiersin.org the combined level of cell body and neuropil labeling in the boxes.

Results

Conclusion