Abstract
The kinetics of yielder cell formation in host-controlled modification mediated by prophage P1 has been examined when Escherichia coli strains B(P1) or Bc251(P1) are infected with restricted forms of T1, λ, or P2, respectively. Yielder cells are formed by linear kinetics with respect to time for at least 3–5 hours after adsorption and incubation. Therefore logarithmically growing cells can hold potentially productive genomes of restricted phase in check for long periods of time. The majority of yielder cells produce either few or no unrestricted phage. Evidence is presented that the probability of yielder cell formation is greater than the probability of spontaneous induction of prophage P1. Under similar conditions, the relationship between such restricted phage as T1, λ, or P2 and nonpermissive cells seems to be identical.
Published Version
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