Abstract
In experiments with rumen epithelium incubated in vitro the ratio of 3-hydroxybutyrate: acetoacetate produced was similar to the ratio reported for portal blood, and the ratio ketogenesis: oxidized to CO2 of butyrate was also close to values reported in vivo. Ovine and bovine epithelium incubated with butyrate differed significantly by the values of about 12-17 and 4-7 obtained for the ratio of 3-hydroxybutyrate: acetoacetate. Increasing levels of butyrate in the incubation medium resulted in a decreasing proportion of butyrate oxidized to CO2 and an increasing proportion of ketogenesis. The addition of glucose to butyrate in the incubation medium significantly increased the rate of ketogenesis from butyrate by ovine and bovine tissues. The addition of glucose to butyrate in the incubation medium significantly decreased the rate of butyrate oxidation to CO2 by ovine and bovine tissues. The ketogenic effect of glucose was also apparent in perfused rumen epithelium with butyrate at the mucosal side and glucose at the serosal side.
Published Version
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