Abstract
The somatic chromosomes of Guizotia abyssinica (L.f.) Cass. (2n = 30) were studied by aceto-orcein, Giemsa C-banding and silver staining methods. Minor variations in chromosome size and centromeric positions (median/submedian) made it difficult to characterize unhanded chromosomes. After C-banding, each chromosome could be assigned to one of four groups. At late prophase and prometaphase stages, four chromosome pairs could be identified as satellited. The satellites of one pair of chromosomes are very small, poorly staining and usually escape detection. The other three pairs and an unsatellited pair have highly reproducible C-bands. In addition, tiny C-positive dots or bands appear sporadically on several of the chromosomes. A maximum of four, six, and seven nucleoli were observed in microspores, somatic interphase and telophase nuclei, respectively. A maximum of seven silver positive nucleolar chromosomes were identified. Associations of all eight satellited chromosomes with a nucleolus were observed. Examples of unusual nucleolar behaviour are described. These comprise the occurrence of large amounts of nucleolar material on the condensed metaphase chromosomes. Also, some telophase cells contained numerous small silver-positive bodies in nuclei and cytoplasm.
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