Abstract
Oesterhelt and Stoeckenius in 1971 found a pigment in Halobacterium halobium which they called bacteriorhodopsin because it resembles rhodopsin in many aspects. The isomeric configurations of its chromophore were studied by thin layer chromatography of the retinal and of the retinal oxime derivatives. The dark form of bacteriorhodopsin (R 560) contains 13- cis retinal, in contrast to the 11- cis retinal of rhodopsin. Both the R 570 and the M 412 forms of bacteriorhodopsin were found to contain all- trans retinal. Implications of the different isomeric forms found in bacteriorhodopsin are discussed.
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