Abstract
Angiotensin-converting enzyme (ACE) is an Zn(II)-containing dipeptidyl carboxypeptidase that converts angiotensin I to the potent vasoconstrictor, angiotensin II. Using oligonucleotide probes based on the amino acid sequence of mouse kidney ACE, cDNA encoding this protein has been isolated. One cDNA, ACE.31, encodes the N-terminal 332 amino acids of mouse ACE, a portion of the protein containing a putative 34-amino acid leader sequence and the N terminus of the mature protein. Northern analyses with cloned ACE cDNA revealed that both mouse kidney and lung express two ACE mRNAs, one of 4900 and another of 4150 bases. Southern analysis suggests that cDNA ACE.31 is the product of a single gene, and thus these data add evidence to the hypothesis that the converting enzymes produced by epithelial and endothelial cells are identical.
Highlights
Southern analysis suggests that cDNA angiotensin-converting enzyme (ACE).31 is the Amino Acid Sequence Analysis-Amino-terminal sequence deterproduct of a single gene, and these data add evi- minations of ACEtryptic peptides were performed using the standard dence to the hypothesis that the converting enzymes produced by epithelial and endothelial cells are identical
37.21,43.9, and 50.53, identified 26 plaques containing cloned cDNA ranging in size from 2800 to 4700 base pairs
The amino acids conserved between neutral endopeptidase and thermolysin and implicated in the active sites of these enzymes are not encoded by ACE.31 they may be found in ACE cDNA encoding further 3'
Summary
Angiotensin-converting enzyme (EC 3.4.15.1) is a Zn(I1)containing dipeptidyl carboxypeptidase produced by many tissues including renal tubular epithelium, ciliated gut epithe-. From the Departments of Pathology, Medicine,and llBiology, Emory University, Atlanta, Georgia 30322 and the §National Institute of Mental Health, Alcohol Abuse, and Mental HealthAdministration and the 11Renal Cell lium, stimulated macrophages, testis, and areas of the brain [7, 8]. It is the production and placement of ACE on the luminal surface of vascular endothelium that is thought to be most significant to pressure regulation [10,11,12,13]. Revealedthat both mouse kidney and luenxgpress two ACE mRNAs, one of4900 and another of 4150 bases
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