Abstract
Forensic DNA laboratories need to provide results in a very short time. Therefore, in addition to crucial quality parameters (e.g., sensitivity and robustness), speed is an increasingly important feature of STR PCR assays. QIAGEN has developed the Investigator ESSplex Plus Kit, which combines the features necessary for fast and reliable analysis of demanding forensic samples. Based on our fast-cycling multiplex PCR technology, we have introduced a novel reaction mix that enables a standard 30 cycle amplification in ∼90 min, setting a new level of speed in STR analysis. Well-balanced full profiles are reliably obtained with 125 pg of DNA template. Even a single genomic DNA copy gives rise to peak heights easily detectable with commonly used analysis thresholds. The assay is very robust and can tolerate PCR inhibitors of up to 200 ng/μl humic acid or 1000 μM hematin without allelic dropout, higher than any other kit on the market. The Investigator ESSplex Plus Kit provides a clean baseline, without artifacts that may interfere with the analysis of low-copy-number samples. These features help to reduce the number of samples that require reanalysis and contribute to more streamlined and efficient laboratory workflow.
Published Version
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