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Abstract
In this study, the role of CoQ10 and berberine in the treatment of ischemia-reperfusion injury is researched by analyzing the biochemical effects of CoQ10 and berberine administered after experimental ischemia-reperfusion injury in rats. 32 Sprague-Dawley 200-250 g male rats were randomly divided into four equal groups as weighting, including: control, ischemia-reperfusion (I/R) and experimental groups treated with CoQ10 (10 mg/kg dose, 3 times with 8 h intervals) and berberine (200 mg/kg dose, 3 times with 8 h intervals) that were administered by intragastric way to the I/R+ CoQ10 and I/R+ berberine group before two hours ischemia two hours reperfusion with tourniquet. In the sham group, only gastrocnemius muscle were removed and given no CoQ10 and Berberine. By the completion of reperfusion; all rats were anesthetized with ketamine and xylazine, sacrificed after taking samples to measure levels of blood lactate dehydrogenase (LDH), creatine kinase (CK); MDA, MPO, GSH, SOD, GPx and CAT in blood and muscle tissues. Compared to control group, ischemic-reperfusion injury significantly increased plasma LDH, CK; MDA and MPO levels, decreased GSH, SOD, GPx and CAT activities of blood and muscle tissues. Treatments groups showed significantly decreased MDA and MPO levels, increased GSH levels, SOD, GPx and CAT activities compared to the IR group. Our results suggest that CoQ10 and Berberine may protect or treat against oxidative damage on ischemia-reperfusion injury and that can be benefical treating association with lower extremity ischemia-reperfusion injury.
Highlights
Ischemia is one of the most widespread damage that are mostly caused by decreased blood flow to an organ various reasons such as transplantation, thrombosis, surgical procedures, hypovolemia, energy production stopping and lack of oxygen and nutrients that occurs in vascular substrates of the tissues [1]
It was found that plasma GSH levels, Glutathione peroxidase (GPx), Superoxide dismutase (SOD) and CAT activities were increased by treatment of Coenzyme Q10 (CoQ10) and Berberine compared with the IR group
Muscle tissue GSH levels, GPx, SOD and CAT activities significantly reduced in the IR group compared with control group
Summary
Ischemia is one of the most widespread damage that are mostly caused by decreased blood flow to an organ various reasons such as transplantation, thrombosis, surgical procedures, hypovolemia, energy production stopping and lack of oxygen and nutrients that occurs in vascular substrates of the tissues [1]. While reconstituting blood flow to tissues in an ischemia may contribute to their survival, it may cause simultaneous damage during reperfusion. This is known as ischemia-reperfusion (I/R) injury. Coenzyme Q10 (CoQ10) is a vitamin like nutrient which known as ubidecarenone or ubiquinone and lipid-soluble compound. It is found in the mitochondria, cell mebranes, lipoproteins in blood and all tissues’cell in the body. CoQ10’s primary role in cellular energy production, the electron transport chain, along the inner mitochondrial membrane. It has been considered that CoQ10 may be effective in treatment particular pathological events as an antioxidant supplementation
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