Abstract
Objective To verify BMP-2 and VEGF gene activated nanobone paste can effectively enhance the vertebral bone of ovariectomized goat. Methods From January, 2011 to May, 2016, the goats had been neutered by ovariectomy 6 months earlier to induceosteoporosis. Then surgery to established the model of vertebral bone defected with nanobone implanting, and the operation vertebrae divided randomly into 3 groups: control group, nanobone group and double gene activated nanobone group. Three months after operation the goats were sacrificed and removed the vertebrae. Micro CT analysis of micro three-dimensional structure of trabecular bone, scanning electron microscope (SEM) analysis of the two-dimensional structure of the vertebrae, the structure of trabecular bone was evaluated by movat pentachrome staining. Results The dual gene activated nanobone group compared with the nanobone group, the bone volume fraction (BV/TV) significantly increased (85% at 1.2 mm vs 43% at 1.2 mm, P < 0.05); the dual gene activated nanobone group compared with nanobone group, in the largest ROI (1.2 mm), TbTh increased 10.9% (374 ± 26.2 μm vs 337 ± 22.3 μm,P < 0.05); Trabecular distribution coefficient (TbPf) was significantly decreased (7.519 ± 0.184 mm-1 vs 7.529 ± 0.261 mm-1, P < 0.05); In the largest ROI (0.8 mm), trabecular distribution coefficient (TbPf) was significantly decreased (283 ± 36.4 μm vs 327 ± 31.2 μm, P < 0.05), In the largest ROI (0.8 mm), the trabecular bone volume (Tbn) was increased 20%(1.404 ± 0.283 mm-1vs 1.173 ± 0.224 mm-1, P < 0.05); Cortical thickness over the implantation area showed asignificant increase of 43% in vertebrae(P < 0.05); The histological analysis revealed a more extensive osseointegration of the dual gene activated nanobone group, with the presence of anabundant osteoid tissue and an osteoblastic celllining. Conclusion BMP-2 and VEGF gene activated nano bone paste can effectively enhance the vertebral bone of ovariectomized goat. Key words: BMP-2; VEGF; Nanobone; Ovariectomized; Goat; Vertebrae
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