Different filling materials in the repair of bone defects in sheep vertebrae with characteristics of the bone absorption and formation

Abstract

Objective To observe the characteristics of the bone absorption and formation with different filling material in the repair of bone defects in sheep vertebral body.Methods In 6 adult healthy goats,the L1-6 vertebral bodies were divided into four groups:sheep allogeneic bone group,acrylic bone cement group,and Cem-OsteticTM artificial bone group.T12 vertebral body was not filled as a blank control.Two goats at 4th,8th and 12th week were executed respectively,the vertebral bodies were removed to prepare the decalcified tissue sections,and the hematoxylin and eosin (HE) staining was done for histological observation.Results By gross observation,sheep pedicle depth was (13.1 ± 1.3) mm,diameter (16.9 ± 1.5) mm,sagittal diameter (12.8-± 1.3) mm,and vertebral height (29.8-± 1.6) mm.All animals survived.At 12th week,the fibrous connective tissue structure could be seen,and none of trabecular bone structure was found histologically in blank control group.In sheep allogeneic bone group,there was significant absorption in the defects,and there were a large number of osteoblasts and trabeculae bone.In acrylic bone cement group,the cement-trabecular bone interface showed a large number of defects which were surrounded by a large number of inflammatory cells.In the Cem-OsteticTM artificial bone group,scattered trabeculae were seen in the defects,which were surrounded by a large number of osteoblasts and osteoclasts.Conclusion Except acrylic bone cement,biodegradation and osteogenesis can be seen in the defects of vertebral bodies filled with other two kinds of materials.Moreover,the obsteogenesis of the allograft bone is more significant. Key words: Goats ; Spinal fractures ; Filling material; Histology