Abstract
Background: Tubulin is known as a heterodimer protein, which includes alpha and beta tubulin subunits. This structural protein plays important roles in pathogenesis and healing different diseases. Biomarkers help in fast and accurate detection of cancer. Proteomic studies can be useful both in biological and clinical research, also help obtain protein expression profiles by using twodimensional electrophoresis, mass spectrometry, and bioinformatics tools. Finding candidate proteins as cancer biomarkers is an interesting area in proteomic investigations. Methods: In the present study, the total protein content of healthy cells of the brain and brain tumor cells were extracted, purified and quantified by Bradford assay. Two-dimensional electrophoresis used for protein separation followed by statistical analysis. Primary protein detection was performed based on the differences in isoelectric pH, the molecular weight of proteins and protein data banks, which was further confirmed by Matrix Assisted Laser Desorption Ionisation-Time-of-Flight (MALDITOF-TOF). Results: In this study, an alpha-tubulin expression found changed (overexpression) in Oligodendroglioma tumors comparing control identified by proteomics analysis. Also, alphatubulin position showed in the oligodendroglioma tumors cluster diagram. Conclusion: Proteome analysis approach has allowed biology and medical studies. Alpha-tubulin introduced as a candidate biomarker for the diagnosis and prediction of oligodendroglioma tumors.
Highlights
We investigated the alpha-tubulin expression change in oligodendroglioma tumor
The protein molecular mechanisms involved in rabies pathogenesis different proteomics approach has used including proteome analysis of several in vitro, and in vivo host models infected with
Phosphorylation and nuclear localization of protein cells involved in cancer may be clinical potential cancer biomarkers.[28,38]. Changes after translation such as acetylation and phosphorylation for tubulin is affecting the variety of diseases
Summary
Oligodendroglioma, a subtype of gliomas, account for 4.2% of all primary brain tumors.[1,2] Gliomas are the most malignant of human brain tumors, and the most common type of primary brain tumor.[3,4,5,6] Oligodendroglioma tumors have a high sensitivity to chemotherapy; usually, they respond positively to the treatment.[7,8] The relevant genes and proteins conveying the favorable clinical effects associated with of 1p, 19q and oligodendroglioma tumors are still unknown.[9,10,11]Tubulin is a heterodimer protein (100 Da), consisting of microtubules (alpha and beta) polypeptide chains. Alpha tubulin has 6 subunit genes, and beta-tubulin has seven subunit genes, that expressed in different types of tissues.[12,13] in the nucleation of microtubules in a eukaryotic cell, γ-tubulin has a function. Tubulin is known as a heterodimer protein, which includes alpha and beta tubulin subunits. This structural protein plays important roles in pathogenesis and healing different diseases. Proteomic studies can be useful both in biological and clinical research, help obtain protein expression profiles by using twodimensional electrophoresis, mass spectrometry, and bioinformatics tools. Results: In this study, an alpha-tubulin expression found changed (overexpression) in Oligodendroglioma tumors comparing control identified by proteomics analysis. Alpha-tubulin introduced as a candidate biomarker for the diagnosis and prediction of oligodendroglioma tumors.
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