Abstract
Background: Proteomics is considered a new era in neurophysiological/ neuropathological research including brain tumors. Gliomas which are derived from glial cells are the most common type of brain tumor in humans. Methods: In the present study the total protein content of healthy cells of the brain and brain tumor cells was extracted, purified and quantified by Bradford assay. Two-dimensional electrophoresis were used for protein separation followed by statistical analysis. Primary protein detection was performed based on the differences in isoelectric pH, molecular weight of proteins and protein data banks, which was further confirmed by MALDI-TOF/TOF mass spectrometry (MS). Results: Our results showed elevated levels of beta-actin protein expression in glioma brain tumor cells. It is important to know when a cell is transformed and when it becomes malignant. Here we evaluated the beta-actin expression in malignant cells. Conclusion: Since structural changes are highly involved in tumor cell malignancy, beta-actin elevations can contribute in glioma tumor cell invasiveness.
Highlights
Cancer is defined as an abnormal growth and replication of cells, which interferes with normal physiology and function of the tissues and the body.[1]
Materials and Methods Twelve malignant human glioma tumor tissues were collected from excess tumor after surgical resection at hospital and tumors grade and malignancy was determined by the pathology department at the same hospital
Twelve spots corresponding to beta-actin were identified with elevated protein expression compared to normal tissue
Summary
Cancer is defined as an abnormal growth and replication of cells, which interferes with normal physiology and function of the tissues and the body.[1]. Primary brain tumors are classified as benign and malignant. Benign tumor cells have a slow proliferation rate, are rarely invasive with frequently normal microscopic phenotype. Malignant brain tumors on the other hand, are invasive with high proliferation rate and abnormal phenotype. Another classification by WHO consists of four tumor grades (I, II, III and IV).[5,6,7]. Results: Our results showed elevated levels of beta-actin protein expression in glioma brain tumor cells. We evaluated the beta-actin expression in malignant cells. Conclusion: Since structural changes are highly involved in tumor cell malignancy, beta-actin elevations can contribute in glioma tumor cell invasiveness.
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