The intestinal mucosal immunity in mice enhanced by crosslinked ready-to-eat sea cucumber via regulating the SIgA secretion and gut microbiota

Abstract

The protective effects of molecular crosslinked ready-to-eat sea cucumber (RSC) against immunosuppression induced by cyclophosphamide in mice were investigated. After gavage of molecular crosslinked RSC, the SIgA levels of intestinal mucosa in mice were significantly increased by 31.6% (P < 0.01), and the terminal differentiation of IgA+ B cell was promoted with the significant up-regulation of IL-6 and IL-10 levels (P < 0.05). Moreover, the secretion mechanism of plasma cell was mainly promoted through XBP-1s pathway mediated by IRE1 (P < 0.01), rather than ATF-4 and ATF-6 pathway (P > 0.05). In addition, the diversity of intestinal flora was increased, and the imbalance of intestinal flora was improved. The Lachnospiraceae_NK4A136_group dominated in crosslinked group could promote the repair of intestinal mucosa. Therefore, molecular crosslinked RSC can alleviate the intestinal mucosal damage via promoting SIgA secretion and regulating gut microbiota of mice.