The interactive effect of an AM fungus and an organic amendment with regard to improving inoculum potential and the growth and nutrition of Trifolium repens in Cd-contaminated soils

Abstract

Aspergillus niger-treated dry olive cake (DryOC) can be used as a soil organic amendment and the aim of this work was to study the effectiveness of this amendment and a Cd-adapted arbuscular mycorrhizal (AM) fungus in improving Trifolium repens growth and nutrition in Cd-contaminated soil. In a compartmentalized growth system, consisting of a root compartment (RC) and two hyphal compartments (HCs), we investigated the influence of the amendment on intraradical and extraradical AM fungi development. In addition, we studied the viability and infectivity of the detached extraradical mycelium in plants, designated as receptor plants, grown in the HC after removal of the RC. Both the amendment and the AM fungus increased shoot and root biomass and nodulation in both the non-contaminated and Cd-contaminated soils. The positive interaction between the microbiologically treated DryOC and the AM fungus resulted in the highest plant yield, which can be explained by enhanced nutrient acquisition and arbuscular richness as well as by the immobilisation of Cd in amended soils. However, A. niger-treated DryOC had no effect on the extraradical mycorrhizal mycelium development. Although Cd decreased AM hyphal length density, symbiotic infectivity was similar in receptor plants grown in non-contaminated and contaminated soil, thus confirming the AM fungal inoculum potential. The combination of the AM fungus and A. niger-treated DryOC increased plant tolerance to Cd in terms of plant growth and nutrition and can be regarded as an important strategy for reclaiming Cd-contaminated soils.