Meadow degradation reduces microbial β diversity and network complexity while enhancing network stability

Abstract

Continuing meadow degradation under the dual impacts of climate change and human activities has altered microbially-mediated biogeochemical cycles. However, microbial diversity and network stability in response to meadow degradation and their relationships to environmental variables remain insufficiently understood. This study focused on alpine meadows with varying degrees of degradation in the hinterland of the Qinghai-Tibetan Plateau. It analyzed the soil microbial diversity (α and β diversity) and ecological network topology characteristics under the degradation of alpine meadows. Furthermore, the extent to which these factors elucidate the environmental changes observed during degradation was explored. The results demonstrated that: (1) the α diversity of soil microbial under degradation of alpine meadows exhibited an increasing trend, although not significantly. The β diversity (community dissimilarity) exhibited a significant decreasing trend. These indicated that meadow degradation resulted in microbial homogenization intra- and inter-community. In the light degradation stage, the β diversity of bacteria and fungi significantly increased with geographic and environmental distance rise. Only bacterial β diversity significantly increased with geographical distance in the moderate degradation stage and environmental distance in the heavy degradation stage, respectively. This indicated that meadow degradation possibly altered microbial assembly, especially for fungi. (2) The degree of soil bacterial nodes exhibited a significant decreasing trend with degradation and fungal eigencentrality demonstrated a significant decreasing trend. After removing the identical nodes, the natural connectivity of the fungal network exhibited a significant decreasing trend with degradation. This suggested that the stability of the soil microbial network increased while the network complexity decreased under meadow degradation. These changes possibly marked the irreversible course of alpine meadow degradation. (3) The Mantel test indicated that bacterial diversity and network topological features were more strongly associated with environmental factors than those of fungi. Akaike information criterion (AIC) values and the upset matrix plots of variance partitioning based on Redundancy analysis (RDA) indicated that higher rates of soil factors, such as soil pH, organic carbon (SOC) content, and total potassium (TK), elucidated changes in microbial community diversity and network topological features. This study highlights the importance of soil microorganisms for ecological stability and the role of microorganisms should be emphasized in future efforts to restore alpine grasslands.