Abstract
Iodate oxidation of pyrogallol at pH 4 produces an agent capable of precipitation poly-l-lysine and lysozyme. This substance is neither purpurogallin nor one of its oxidation products. The solubility of the poly-l-lysine precipitate in 6m-guanidine hydrochloride and in 1m-NaOH indicates that hydrogen bonding is responsible for precipitation. While the oxidized pyrogallol solutions do not contain tannins detectable by the method ofChapon, polyvinylpyrollidone (PVP) does prevent precipitation. Once formed, the precipitate does not redissolve in PVP. The pyrogallol oxidation products have no effect on the lysine-free polypeptide (poly-N(5) hydroxyethyl-l-glutamine.
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