The interaction between Glut4 and IL-1β gene polymorphisms and risk factors of obstructive sleep apnea syndrome

Abstract

Objective To investigate the interaction between Glut4 and IL-1β gene polymorphisms and risk factors of obstructive sleep apnea syndrome (OSAS) in development of OSAS by using multifactorial dimension reduction (MDR) . Methods From January to December in 2010, consecutive patients hospitalized in the Hypertension Department of Xinjiang Autonomous Region People's Hospital were included in this study. Night-time polysomnography and biochemical indicators were measured in 606 hypertensive patients suspected to have OSAS (including 447 cases of confirmed OSAS) . Taqman-PCR was used to genotype the taq-SNPs of Glut4 (rs5417, rs5415, rs5435) and IL-1β (rs1143627, rs1143634, rs1143633) . The interactions between glut4 and IL-1β SNP locus polymorphisms and risk factors of OSAS (such as obesity) were analyzed. Results There was no significant difference in population distribution and model construction for Glut4 rs5417 and IL-1 rs1143633 genotypes between OSAS and control groups. The frequency of CC genotype at Glut4 rs5417 locus was significantly higher in the OSAS group than that in the control group (63.3% vs 27.7%, P<0.05) . After adjustment of sex, age, triglyceride, cholesterol, blood glucose, body mass index and other risk factors, Logistic regression analysis showed that the synergistic effect of the Glut4 Rs5417 and IL-1β rs1143633 significantly increased the risk for obstructive sleep apnea in the Han population [OR (95%CI) : 0.5 (0.3, 0.9) ] (P=0.012) . In the overweight and obese patients, GST4 rs5417 (CC) genotype and IL-1β rs1143633 (CT) genotype increased the risk for OSAS [OR (95%CI) : 4.9 (2.8, 8.5) , P<0.05; OR (95%CI) 4.4 (2.3, 8.6) , P<0.05 respectively]. The optimal model of MDR data showed a strong synergy between the Glut4 rs5417 and IL-1β rs1143633, with the test accuracy being 57. 0, and a good consistency in cross-test (8/10) (P=0.004) . Conclusion During the development and progression of OSAS, synergistic interactions between the Glut4 rs5417 and IL-1β rs1143633 may increase the risk of OSAS. Overweight and obese patients who carry rs5417 (CC) and/or rs1143633 (CT) may have synergistically increased risk for OSAS. Key words: Sleep apnea, obstructive; Multifactorial dimension reduction; Glut4; IL-1β; Interaction