Abstract
The main objective of the study was to determine the antibacterial effect of nicotine against the Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Specifically, it determined the bacterium that is resistant and susceptible in nicotine and the significant difference in the inhibitory effect of Nicotine on E. coli and S. aureus . Experimental design was used subjecting both bacteria to the nicotine extract, and their susceptibility was measured by the diameter of the zone of inhibition in millimeter. The finding revealed that S. aureus is found to be susceptible to nicotine extract. On the other hand, the extract has an intermediate effect on E. coli . Hence, nicotine can be further studied for its medicinal property. Moreover, there is a significant difference in the inhibitory effect of nicotine against S. aureus and E. coli . Thus, the null hypothesis is rejected. Â Keywords: Nicotine, antibacterial effect, Staphylococcus aureus , Escherichia coli
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