Abstract
The present study was aimed to evaluate the antioxidant potential and inhibitory effect ofCannabis sativa and Morus nigra against lipid peroxidation in goat brain and liver homogenates. The formation of free radicals, highly reactive oxygen species (ROS) and reactive nitrogen species (RNS) is a normal metabolic process for cellular signaling and countering the antigens. However, they may cause serious damage if they produced at amplified tolls. In addition, metabolic disorders also serve as sources of these reactive species. Although the issue can be addressed through supplements and other phytochemicals. In this study, two plant species were evaluated for their biological potential by employing a spectrum of antioxidant assays. The antioxidant activity was performed by lipid peroxidation assay. The water extract prepared from leaves of Cannabis sativa and Morus nigra showed significant (P<0.05) inhibition as compared to control i.e., 522.6±0.06 and 659.97±0.03 µg/mL against iron-induced lipid peroxidation in goat brain homogenate while the inhibitions were 273.54±0.04 and 309.18±0.05 µg/mL against nitroprusside induced lipid peroxidation of the brain. The iron and nitroprusside induced lipid peroxidation was also significantly inhibited by leaf extracts of Cannabis sativa and Morus nigra in liver homogenates such as 230.63±0.52 and 326.91±0.01 µg/mL (iron-induced) while 300.47±0.07 and 300.47±0.07 µg/mL (nitroprusside induced), respectively. The extracts of Cannabis sativa extract showed promising activity (96.04±0.060%) against DPPH radicals while Morus nigra showed a moderate activity (34.11±0.120%). The results suggest that different accessions ofCannabis sativa and Morus nigra are a potential source of antioxidants and have a therapeutic effect against disease induced by oxidative stress and hence can be used for novel drug discovery and development.
Highlights
The medicinal plants are of the main interest for medicines and scientific research and many important agents having medicinal importance were being isolated from these plants (Rana et al, 2021)
The highest antioxidant activity was observed at 96.04% for C. sativa leaf extract and 34.11% for M. nigra at 400 μg /mL while the least antioxidant activity was for extracts was observed at 25 μg /mL (24.18 and 19.14%, respectively)
One of the objectives of this study was to evaluate the protective effect of C. sativa and M. nigra against lipid peroxidation which is a malicious metabolic process observed in a variety of diseases, the results are summarized in Figure 3 and 4
Summary
The medicinal plants are of the main interest for medicines and scientific research and many important agents having medicinal importance were being isolated from these plants (Rana et al, 2021). The research teams have isolated many important substances from these plants for the formulation of new medicines. Phytochemicals originated from plants that are mostly non-nutritional but control many important functions in human beings. The estimated number of superior plants which includes angiosperms and gymnosperms are 250000 and this potential source of the plants is largely unexploited and only 6% were evaluated for their biological activities and out of these only 15% were estimated on the phytochemical plant (Faria et al, 2021). We are being challenged by the revival of new diseases mainly those which are caused by oxidative stress. Synthetic antioxidants can cause toxicological problems so the research was progressed to isolate the plant’s based natural antioxidants (Sabir et al, 2020)
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