Abstract
Abstract The effect of 3,4-dihydroxybutyl-1-phosphonate on phosphoglyceride metabolism of Escherichia coli strain 8 was examined. The compound inhibits the incorporation of [33P]phosphate into phospholipids. Phosphatidylglycerol accumulation is very strongly inhibited, while phosphatidylethanolamine and cardiolipin accumulation are less significantly affected. Pulse labeling studies using [32P]phosphate indicate that phospholipid synthesis is inhibited to a much greater extent by 3,4-dihydroxybutyl-1-phosphonate than is the synthesis of DNA or RNA. These studies also indicate that 3,4-dihydroxybutyl-1-phosphonate severely inhibits phosphatidylglycerol synthesis and that phosphatidylethanolamine synthesis is inhibited less severely. Cardiolipin synthesis is only slightly affected. Studies on the rate of disappearance of [32P]phosphate from the phosphoglycerides of prelabeled cells indicate that 3,4-dihydroxybutyl-1-phosphonate treatment does not alter the rate of turnover of the phospholipids. 3,4-Dihydroxy[3-3H]butyl-1-phosphonate was readily incorporated into a chloroform-extractable fraction of E. coli strain 8.
Highlights
-phosphonate than is the synthesis of DNA or RNA
Studies on the rate of disappearance of [32P]phosphate from the phosphoglycerides of prelabeled cells indicate that 3,4-dihydroxybutyl-1-phosphonate treatment does not alter the rate of turnover of the phospholipids
An analysis of the fates of the individual phospholipids (Fig. 1, B to D) indicates that phosphatidylglycerol accumulation was very strongly inhibited while the accumulation of phosphatidylethanolamine and cardiolipin were much less significantly affected by the addition of 3,4-dihydroxybutyl-I-phosphonate
Summary
The effect of 3,4-dihydroxybutyl-1-phosphonate on phosphoglyceride metabolism of Escherichia coli strain 8 was examined. Pulse labeling studies using [32P]phosphate indicate that phospholipid synthesis is inhibited to a much greater extent by 3,4-dihydroxybutyl-1. Studies on the incorporation of labeled precursors into DNA, RNA, protein, and lipid revealed that the 4-carbon phosphonate inhibits phospholipid synthesis most effectively. E. coli strain 8 with 3,4-dihydroxybutyl-l-phosphonate caused an alteration in the distribution of labeled acetate incorporated into the phospholipid fraction. The major change was a reduction in the percentage of radioactivity in the phosphatidylglycerol fraction Treatment of this strain with glycerol 3-phosphate had little effect on the distribution of label into the phosphoglycerides. The present report describes further studies on the role played by 3,4-dihydroxybutyl-1-phosphonate on phospholipid metabolism in E. coli strain 8. The 4-carbon phosphonate was discovered to inhibit the growth of E. coli strains possessing an active glycerol 3-phosphate transport system [2].
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