Abstract
1. The sulphation of polyethyleneglycol 200 by the isolated perfused guinea pig liver is inhibited to about 60% by 10 mM ClO3- in the plasma of the perfusate when the concentration of SO4(2-) therein is 1.18 mM. 2. The inhibition is almost complete when the concentration of SO4(2-) is about 0.1 mM, a level which can be achieved by using a modified Ringer-bicarbonate solution, devoid of sulphate, to prepare the perfusate. 3. Chlorate, presumably through its action on ATP-sulphurylase, may therefore be a useful inhibitor of sulphation in the isolated perfused liver when the activity of the sulphurylase is rate-limiting. 4. The rate of bile production in the presence of chlorate is no different from that in its absence showing that, in the time scale of the perfusion, chlorate is not a general liver poison. 5. When the synthesis of PAPS is not rate-limiting, as in the sulphation of oestrone metabolites by rat liver, chlorate has no effect on the rate of sulphation.
Published Version
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