The inhibition ability of copper nanoparticles synthesized by green tea extract on healing the induced contaminated wounds in laboratory hamsters

Abstract

The research was carried out in order to synthesize CuNPs which use extract of green tea, then characterize them and investigate its antibacterial only or in combination with Ciprofloxacin for the treatment of S. aureus. Then, use a blood test to verify its efficacy in healing wounds infected with S. aureus in laboratory Hamsters.The results showed that green tea was successful in converting copper nitrate to CuNPs, as evidenced by the ultraviolet spectrum, which showed an absorbance at 271 nm. CuNPs with a size range of 5–65 nm and an average size of 5–20 nm were found to have a spherical form. CuNPs had a 20% antibacterial and a synergistic effect when mixed with Ciprofloxacin against S. aureus. It appeared that the CuNPs or ciprofloxacin, alone or in combination, had synergistic effects in healing the infected wounds in a shorter length of time than the control group of treatment in the biological study. The use of S. aureus to heal induced wounds resulted in significantly higher WBCs and lower platelet counts, whereas the treatment with CuNPs or Ciprofloxacin, alone or in combination, resulted in WBCs and platelet counts that were referred within normal ranges and did not differ from the control group. Furthermore, appeared non differences in Hb, RBCs, or PCV, urea, creatinine as well as the levels of IgG, IgA antibody and the liver enzyme of GOT and GPT across all treatment groups compared with the values in infection animal groups.