Abstract
Although interference microscopy indicates that onion epidermal cells adjacent to cut margins decline in NDM (nuclear dry mass) by an average of 48%, the present study indicates no differences among nuclei beyond the nucleus of the second cell. Therefore, only nuclei of the extreme margin of cut tissue or internal nuclei exhibiting pycnotic symptoms (perhaps due to handling) need be avoided in sampling. This permits a greater proportion of a piece of tissue to be used in experimentation than was formerly considered feasible (Kulfinski and Pappelis 1975).
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