Abstract
The aim of this study was to evaluate the proliferation rate and morphological changes of adipose-derived mesenchymal stem cells of canine and equine origin (Eq- and CaAdMSC). Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. Proliferation activity of cells was determined with the Alamar Blue assay. Obtained results, normalized in respect to the control culture, showed that EqAdMSC exposed to MF maintained a high proliferation status, whereas proliferation activity of CaAdMSC cultured in the presence of MF was decreased. Estimations of population doubling time (PDT) also revealed that EqAdMSCs exposed to static MF achieved a twofold increase in the total number of cells in a shorter amount of time than the control culture. The PDT value obtained for investigated CaAdMSCs indicated that MF exposure resulted in the prolongation of population doubling time. Morphology of cells and cellular composition was investigated using a light inverted microscope and a fluorescent microscope. A scanning electron microscope was used for microvesicles (MVs) imaging. Obtained results showed that both cell types maintained fibroblastic morphology and did not reveal signs of apoptosis or necrosis. However, the MF had an influence on the MVs secretion. While EqAdMSCs propagated in the presence of MF were characterized by the abundant MVs presence, CaAdMSCs revealed poor secretory activity. The approach presented provides complex analysis, which enables one to determine changes in equine and canine cytophysiology.
Highlights
IntroductionDegenerative disorders of the musculoskeletal system are characterized by high occurrence, and affects both large and small animals (Butt 2002; Mohammed et al 2007)
The injuries of bones and cartilages are serious problems for veterinary medicine
The growth rate of AdMSCs was evaluated for 7 d, both in the presence as well as in the absence of static magnetic field (MF)
Summary
Degenerative disorders of the musculoskeletal system are characterized by high occurrence, and affects both large and small animals (Butt 2002; Mohammed et al 2007) To overcome this issue, many approaches are applied. Treatment options mainly involve the utilization of two classes of drugs; non-steroidal anti-inflammatory drugs (NSAIDs), and glucocorticosteroids (i.e., steroid drugs) These groups of drugs help to control pain and inflammation associated with tissue degeneration. Therapies with autologous and/or allogeneic transplantations of mesenchymal stem cells (MSC) seem to be the solution for this problem. The goal of this approach is to trigger the healing process and restore tissue functionality. Injections with MSCs are widely used for treating locomotive disorders and have shown many positive clinical results, confirmed with long-term follow-up studies (Marycz et al 2012a; 2012b)
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