An In Vitro Analysis of Pattern Cell Migration of Equine Adipose Derived Mesenchymal Stem Cells (EqASCs) Using Iron Oxide Nanoparticles (IO) in Static Magnetic Field

Abstract

The aim of this work was to investigate the effects of various combinations of a static magnetic field and iron oxide nanoparticles on the growth, proliferation rate, morphology and ultrastructure of equine adipose-derived mesenchymal stem cells (ASCs). Cell migration patterns were also evaluated in order to determine whether the stem cells could be attracted using a magnetic field after iron oxide uptake. Proliferation activity of cells after magnetic field and iron oxide treatment was determined with a resazurin based cytotoxic assay whereas morphology, cytophisiological activity and ultrastructure were evaluated utilizing light, fluorescent and scanning electron microscopy. Morphological and ultrastructural examination of equine ASCs showed that exposure to a magnetic field did not cause any significant changes in cell morphology, however a shift of nuclei to the peripheral parts of the cell was observed in ultrastructural examinations. Although the cells had a lower proliferation factor after the uptake of iron nanoparticles, they could still be attracted to the desired area using a static magnetic field. This method may allow ASCs to be directed and retained within the desired area. These findings support the potential value of the combination of static magnetic fields and iron oxide nanoparticles in the treatment of equine injures.