The Influence of Static Magnetic Field on Neuronal Outgrowth of PC12 cells

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Previously investigations indicated that pulse electromagnetic field (PEMF) can be a neuroregenerative stimulator for stroke and traumatic injuring patients. It can be a local healing stimulator for nerve regeneration and has potential to become a non-invasive physical therapeutic tool for these patients. Recently, it was found that, similar to PEMF, static magnetic field (SMF) can promote osteoprogenitors differentiate into mature phenotype. Accordingly, in this study proposal, we hypothesize that SMF can also be a non-invasive stimulator to promote nerve regeneration. To test this hypothesis, PC12 cells were carried out for in vitro experiments. The cells were pre-induced to express neural characteristics with 25 ng/ml and 50 ng/ml nerve growth factor (NGF). Then the induced cells were continuously exposed to 4000G SMF for 15 days. Cell morphologies were observed by optical microscope at 5, 10 and 15 days after SMF treatment. The images of the cells were recorded by a digital camera at the observation times. In addition, we compared the morphology changes of the differentiated PC12 cells treated with and without SMF exposure by counting the length and branching degree of dendrites. Our preliminary results showed that SMF-treatment has neurite-elongation effect in the NGF-induced PC12 cells. These results suggest that the SMF may affect neurogenerative process at the second stage, i.e, neurite elongation. When the cells were exposed to SMF, the population of the cells extending neurites of length more than once, twice and thrice the cell diameter, the datum were 94.84±4.51%、74.05±13.09% and 57.04±6.45%, respectively. However, the analogous value of the unexposed cells of the different multiple were taken into account, these results were significantly reduced to 74.76±5.16%、48.52±8.48% and 31.59±11.75% So, base on these findings, additional experiments on the mechanism of such effect should be valuable for more insight into the application of SMF-induced nerve regeneration or nerve differentiation.