The influence of SnoN gene silencing by siRNA on the cell proliferation and apoptosis of human pancreatic cancer cells.

Chengli Liu,Cheng Wang,Yalin Kong,Xiaoxia Zang,Hongyi Zhang,Hui Zhang

doi:10.1186/s13000-015-0267-3

Chengli Liu, Cheng Wang + Show 4 more

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https://doi.org/10.1186/s13000-015-0267-3

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Journal: Diagnostic Pathology	Publication Date: Apr 18, 2015
Citations: 41	License type: CC BY 4.0

Affiliation: Air Force General Hospital PLA

Abstract

BackgroundThe prognosis for pancreatic cancer (PC) is very poor. The SnoN gene may have a role in cell proliferation and apoptosis in human cancer. However, the influence of SnoN on cell proliferation and apoptosis in human PC cells remains unknown.MethodsSnoN expression was assessed in SW1990 PC cell lines using real-time polymerase chain reaction (PCR). A luciferase reporter assay was used to confirm the target associations. The effect of SnoN on cell proliferation in vitro was confirmed using Cell Counting Kit-8. Apoptosis was confirmed using flow cytometry. Gene and protein expression were examined using real time PCR and Western blotting, respectively.ResultsSnoN siRNA significantly inhibited the growth of SW1990 cells by decreasing cell proliferation (P < 0.05) and increasing cell apoptosis (P < 0.05), compared with the blank group and the negative control group. The highest inhibition of cell proliferation appeared at 3 days post-transfection. Cell apoptosis more obvious at 48 h after transfection.ConclusionsIn summary, our results reveal that the RNAi-mediated downregulation of SnoN effectively inhibited the proliferation of PC cells. SnoN-siRNA also enhanced SW1990 PC cell apoptosis. These findings indicate that SnoN gene plays an important role in pancreatic cancer development, and might serve as a potential therapeutic target for pancreatic cancer. However, further in vivo studies are needed to clarify the influence of SnoN gene silencing by siRNA on pancreatic cancer therapy.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7609324661510147

Highlights

The prognosis for pancreatic cancer (PC) is very poor
A number of studies [5,6,7,8] have showed that the development and progression of Pancreatic cancer (PC) are linked with complex gene regulation, such as the inactivation of tumour suppressor genes, the activation of proto-oncogenes, abnormal regulation of cell proliferation and apoptosis adjustment disorders, and abnormal expression of growth factors and their receptors
The results after real-time polymerase chain reaction (PCR) analysis showed little difference in band brightness between the five β-actin groups, which revealed that the RNA template for reverse transcription was equal in each group

Summary

Introduction

The prognosis for pancreatic cancer (PC) is very poor. The SnoN gene may have a role in cell proliferation and apoptosis in human cancer. The influence of SnoN on cell proliferation and apoptosis in human PC cells remains unknown. The incidence of PC is lower than that of many other types of cancer. Because of nonspecific incipient symptoms and early metastasis, PC is highly malignant and invasive, resulting in poor prognosis [2]. A number of studies [5,6,7,8] have showed that the development and progression of PC are linked with complex gene regulation, such as the inactivation of tumour suppressor genes, the activation of proto-oncogenes, abnormal regulation of cell proliferation and apoptosis adjustment disorders, and abnormal expression of growth factors and their receptors.

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