Abstract
Using a wide range of different physical and chemical methods, it was found that the oxidative stress caused by addition of hydrogen peroxide to the incubation medium has a significant effect on the conformation of haematoporphyrin, influencing the oxygen-binding properties of haemoglobin in red blood cells. Morphofunctional characteristics of red blood cells change; in particular, we have observed the transformation of erythrocytes, their transition into echinocytes. In erythrocytes, in response to increased lipid peroxidation (LPO) antioxidant enzymes become active. The use of natural antioxidants (β-carotene and resveratrol) works towards reducting the level of oxidative processes. Resveratrol has the greatest antioxidant effect.
Highlights
The core of cells’ functional activities comprises a variety of oxidative processes
We aimed to reveal the influence of oxidative processes on the oxygen transport capacity of erythrocyte haemoglobin and the ability of antioxidants of natural origin to influence the intensity of lipid peroxidation (LPO) and morphometric characteristics of erythrocytes
In our research the intensity of peroxidation processes was judged by the level of primary and secondary products of LPO and TBARS
Summary
The core of cells’ functional activities comprises a variety of oxidative processes. The physiological state of cells depends on the intensity of their course [1]. The main factors that regulate the rate of such processes are the formation of reactive oxygen species (ROS) [9,10,11], as well as the number of antioxidant compounds [12, 13] and the activity of enzymes that ensure their decay [14]. During some pathological conditions, the antioxidant system of erythrocytes is insufficient [15]. The topical issue is the use of natural antioxidants (AO) able to reduce the intensity of oxidative processes, and to neutralise ROS by the aid of the formation of weak compounds that are not capable of continuing the oxidation chain [16]. Natural AOs can play a significant role in the prevention of diseases associated with oxidative damage (e.g., ischaemia), and serve as an effective tool in the prevention of specific pathological processes [11, 17, 18]
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