Abstract
The rate of differentiation and longevity of mouse neuroblastoma cells NIE-115 in culture media of various compositions have been investigated. In serum-free media the dependence of the differentiation period on concentrations of sodium ions, amino acids, and carbohydrates as well as on the pH values had maximum. Differentiated cells exhibited maximal longevity in those media in which the differentiation time was comparable with the duration of the cellular cycle.
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