Abstract
Publisher Summary This chapter describes the influence of fatty acids and membrane lipids on the spin state of purified cytochrome P-450. Cytochrome P-450 contains a significant amount of high-spin component in the membrane of the endoplasmic reticulum, but when the hemoprotein is removed from the membrane by detergents and subsequently purified, cytochrome P-450 exists predominantly in the low-spin configuration. In this study, unsaturated fatty acids are shown to be responsible for most of the high-spin P-450 in the microsomal membrane. The findings suggest that cytochrome P-450 exists predominantly in the high-spin configuration in the membrane of the hepatic endoplasmic reticulum in contrast to the soluble, partially-purified form of the hemoprotein, which exists mainly in the low-spin form. The results show that microsomal lipids and oleic acid in particular are at least partially responsible for the maintenance of the high-spin configuration in the membrane as verified by the influence of both microsomal lipid extracts and authentic, exogenous oleic acid on the spin state of the hemoprotein.
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