The Influence of Epigallocatechin Gallate on the Nuclear Factor Associated T Cell-1 and Sclerostin Expression in Wistar Rats (Rattus novergicus) during the Orthodontic Tooth Movement

Abstract

Orthodontic tooth movement is obtained through alveolar bone remodeling and periodontal tissue in response to the mechanical forces. Epigallocatechin Gallate (EGCG) is the most abundant bioactive component in green tea which is promising therapy for bone remodeling. The aim of the study was to elucidate the effect of EGCG to the expression of Nuclear Factor of Associated T Cells-1 (NFATC1) and sclerostin (SOST) in orthodontic tooth movement animal model. The samples consisted of 28-healthy male wistar rats (Rattus Novergicus) which were randomly selected then divided into 4 groups (n=7): negative control group (K-) without Orthodontic Tooth Movement (OTM) and EGCG; positive control group (K+) with OTM and without EGCG, group (T1) with OTM and EGCG from day 7 to day 14, group (T2) with OTM (14 days) and EGCG for 14 days. The OTM were installed from the labial surfaces of both maxillary central incisor with light force of 10g/mm2 close coil spring connected to the first left maxillary molar. NFATC1 and SOST expression in the alveolar bone was examined by means of Imunohistochemistry. Analysis of Variance (ANOVA) then continued with Tukey HSD (p<0.05) was done. The highest number of NFATc1 and SOST expression was found in the K+ group compression area. There were significant different of NFATc1 and SOST expression in compression and tensile area between groups (p<0.05). Daily oral administration of EGCG for 14 days can reduce the SOST and NFATc1 expression during OTM in compression area and tensile area during OTM.