The influence of endogenous protein complex on morpho‐functional activity of bone marrow cells (616.3)

Abstract

Endogenous regulators of proliferation are considered as a main player of cell‐cycle regulatory system. Specific actions (stimulation or inhibition) on the target cell are the primary function of regulators. Stimulating growth factors are well described while Inhibitory factors are still less known. One of the growth inhibitory endogenous thermostabile complex is identified in various organs of phylogeneticaly distance organisms (from bacteria to human). The complex decreases the homologous cells proliferation by inhibiting of RNA synthesis. The complex is not species specific but it is characterized by organ specificity, which is revealed only on terminally differentiated cells.The Goal Investigation of endogenous protein complex influence on morpho‐functional activity of bone marrow.Objectives Investigations were carried out on growing rats and adult mice. Obtaining of endogenous thermostabile protein complex from various organs of rat with alcohol extraction was performed; The determination of mitotic index was used for estimation of cell proliferation activity.Results We have found that mitotic index has not changed after the injection of endogenous thermostabile protein complex. Particularly colchicine mitotic index of bone marrow cells in adult mice of control group equals to 65±1.3, mitotic index is 63±2.5‰ after the injection of protein complex. The same picture is observed in growing rats. The value of mitotic index is not changed with compared to control group.Conclusion On the bases of our results we can conclude that organ specificity of protein complex, is still maintained in case of bone marrow cells despite that fact that bone marrow contains the cells with different degrees of differentiation.This results may be considered as new evidence about the action of endogenous protein complex and its possible usage in medicine for development of new therapeutic approaches in the treatment of cancer.