Abstract
Introduction. Some causative agents of sapro-zoonotic infections can multiply in the external environment (water reservoirs, soils, plants), as well as in animals, including microorganisms of the genus Yersinia. Material and methods. Isolation and identification of Y. pseudotuberculosis was carried out in accordance with the instructions on “Epidemiology, laboratory diagnosis of yersiniosis, organization and conduct of preventive and anti-epidemiological measures”. Antibiotic sensitivity was performed via the disc diffusion method in accordance with EUCAST and national guidelines. Biofilm formation was tested using the spectrophotometric assay. Results. It was established that the studied cultures showed a decrease in the level of saccharolytic activity during cultivation at a temperature of +37°C in comparison with the results obtained at 25°C, changes in sensitivity to antibiotics depending on the temperature of cultivation were revealed. It was established that Y. pseudotuberculosis cultures were able to form denser (λ 570) biofilms when cultured at 25°C, in comparison with biofilms formed at 37°C. Conclusions. Biological characteristics of the studied Y. pseudotuberculosis isolates (changes in the saccharolytic activity, the level of sensitivity to antibiotics and the formation of biofilms were revealed) depends on the cultivation conditions.
Highlights
Some causative agents of sapro-zoonotic infections can multiply in the external environment, as well as in animals, including microorganisms of the genus Yersinia
The study carried out the Y. pseudotuberculosis isolation and identification in accordance with
Five cultures of Y. pseudotuberculosis were investigated, which have been observed in synanthropic rodents
Summary
Some causative agents of sapro-zoonotic infections can multiply in the external environment (water reservoirs, soils, plants), as well as in animals, including microorganisms of the genus Yersinia. Some causative agents of sapro-zoonotic infections are capable of reproduction in the external environment (water reservoirs, soils, plants), as well as in the body of animals, including microorganisms of the genus Yersinia. The diseasecausing pathogens occurring in humans and animals Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica are quite common. Yersiniosis is a foodborne gastrointestinal tract zoonotic disease that can be transmitted via contaminated food or water. In Ukraine, there are active natural reservoirs of Y. pseudotuberculosis. Contamination of livestock products and/or food causes human diseases. There are evidences, suggesting that Y. pseudotuberculosis serotype O1b has been isolated due to intragenomic rearrangements and deletions of Y
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