The influence of citrates Ag and Cu on the lipid composition of the tissues of the honey bees body and bee bread under the condition of their added to the feeding in the spring period

Abstract

Experimental data of the lipid composition of the honey bees and bee bread in the spring period under the conditions of feeding sugar syrup with Ag and Cu citrates are presented in the article. The studies were conducted on 3 groups of bee colonies, three bee colonies each. Control group (I) received sugar syrup at a concentration of 1:1 (1000 ml per week), experimental (II) group received under similar conditions with sugar syrup 0.2 mg of Argentum and 0.2 mg of Cuprum in citrate form. The third experimental group was fed with sugar syrup Ag and Cu citrates at doses of 0.5 mg each per 1000 ml of sugar syrup per week. The study was lasted 36 days. Samples of tissues of the whole organism of honey bees in the amount of 30–35 pieces were selected for the study. from each bee colony and bee bread to determine the total lipid content by the Folch method and their separate fractions: phospholipids, mono- and diacylglycerols, free cholesterol, non-esterified fatty acids, triacylglycerols, esterified cholesterol glycol cholesterol using thin layer chromatography using silica gel plates Sorbfil. According to the results of the research, we found intergroup differences of total lipid content in the tissues of whole organism of the honey bees and bee bread of experimental groups in comparison with the control group. Higher levels of phospholipids, esterified cholesterol, and lower levels of free cholesterol, mono- and diacylglycerols and triacylglycerols were found in the tissue of bees II and III of the experimental group. Introduction of Ag and Cu citrates into the components of bee feeding in the bee bread the level of phospholipids (P ˂ 0.01), mono and diacylglycerols (group III), triacylglycerols increased against the decrease of free cholesterol (P ˂ 0.05) and esterified cholesterol (P ˂ 0.01).