THE INFLUENCE OF ANTIOXIDANT COMPOUNDS ON THE BIOCHEMICAL INDICATORS OF RATSʼ BLOOD SERUM UNDER ACUTE HEPATITIS

Abstract

Introduction. Hepatitis of various etiologies is a global problem, which is caused by their high socio-economic significance and widespread prevalence among people of working age. One of the actual problems is the search for effective combinations of substances that have antioxidant and hepatoprotective properties. The most common antioxidants used to correct hepatitis include α-lipoic acid, quercetin and vitamins A, E, C. Aim. The aim of the work was to study the effectiveness of using the antioxidant complexes «Triovit» + quercetin and «Triovit» + quercetin + α-lipoic acid under the conditions of acute hepatitis. Methods. Acute hepatitis was caused by single intraperitoneal injection of hydrazine sulfate (100 mg/kg). Antioxidants were injected immediately after the injection of hydrazine sulfate in the following concentrations: "Triovit" - 50 mg / kg; quercetin - 20 mg / kg; α-lipoic acid - 100 mg / kg. The experiment lasted 24 hours. The intensity of free radical processes was determined by the concentration of diene conjugates. The state of hepatocytes was determined by the activity of ALT, AST and bilirubin concentration in serum. Synthetic function of the liver was determined by concentration of cholesterol in serum. Results. The results of the conducted research prove that using the antioxidant complex which contains quercetin and “Triovit” normalizes indexes of free radical processes in liver, promotes recovering aminotransferase and cholesterol indexes, but does not reduce the bilirubin concentration in serum. Using the antioxidant complex which contains “Triovit” + quercetin + α-lipoic acid caused activation of lipid peroxidation processes, decrease in the activity of aminotransferases and cholesterol level. Conclusion. Using the complex of antioxidants "Triovit" + quercetin is more effective in correction of acute hepatitis in comparison with "Triovit" + quercetin + α-lipoic acid.