Lipid peroxidation and antioxidant defense system in patients with kidney cancer

Abstract

Purpose of the study: to assess the state of lipid peroxidation processes in patients with kidney cancer in relation to the clinical factors of the prognosis of the disease and against the background of surgical intervention.Material and methods. For the study, we used blood plasma samples from 300 patients with kidney cancer T1–4N 0–1M0–1, obtained before surgery and 8–9 days after surgery. Oxidative stress was assessed by the intensity of free radical processes by the method of induced biochemiluminescence on the BKhL-07 biochemiluminometer apparatus (Medozons Co., Russia), the activity of lipid peroxidation processes was assessed by the accumulation of initial products in the blood plasma – diene conjugates (DC), triene conjugates (TC) and fial Schiff bases (OSh) according to I. A. Volchegorsky et al. method. (1989). Statistical processing of the results was carried out using Statistica 12 and Microsoft Offie Excel 2010 programs.Results. In patients with kidney cancer, there was a decrease in the level of DC, an increase in OSh and the ratio of Osh / (DC + TC) compared with the control (p ≤ 0.05), against the background of depletion of the resources of the antioxidant defense system. The content of DC did not differ from the norm with the size of the primary tumor of the kidney pT1–pT2 and decreased (p ≤ 0.05) in the case of the presence of the tumor pT3–pT4. At the same time, there was an increase in the OSh content (p ≤ 0.05). In patients with thrombi in the inferior vena cava system, a decrease in the level of DC was observed (p ≤ 0.05), the OSh level did not differ from that of healthy individuals. In the postoperative period, there was an increase in the content of DC (p ≤ 0.05) and a decrease in the Osh / (DC + TC) coeffiient (p ≤ 0.05) compared with the initial level.Conclusion. Kidney cancer is accompanied by the activation of free radical oxidation processes against the background of depletion of the resources of the antioxidant defense system. Changes in the pro-antioxidant homeostasis are interrelated with the size of the primary tumor and the presence of a tumor thrombus. In the postoperative period, the activity of free radical oxidation processes in the patient’s body decreases.