Abstract
Introduction: The purpose of this study was to evaluate and compare the features of the initiation and development of oxidative stress in patients with osteomyelitis and burns.
 Methods: We studied the oxidative metabolism of blood of 20 healthy subjects (controls), 15 patients with burns, and 18 patients with chronic osteomyelitis. All patients included in the second group had thermal burns of the I-II-III degree in trunk and limbs on an area of 31-80% of the body surface without thermal inhalation trauma. After standard sample preparation, a wide range of parameters of oxidative metabolism was determined in the blood. The intensity of free radical processes in blood plasma and red blood cells, and the total antioxidant activity was evaluated by Fe-induced biochemiluminescence. The concentration of malonic dialdehyde in blood plasma and red blood cells was determined. The level of diene and triene conjugates and Schiff bases was determined spectrophotometrically using reagent kits. The catalase and superoxide dismutase activities in the red blood cells of patients from each of the groups was also determined.
 Results: We showed that in osteomyelitis, which is a long-lasting process, changes in the balance of free radical generation and activity of the antioxidant system were compensatory and mostly related to changes in blood plasma. On the contrary, in burn victims, oxidative stress signs had a maladaptive character. They were seen in blood plasma and red blood cells, and accompanied by a pronounced depletion of enzyme antioxidant system reserves.
 Conclusion: Our study demonstrate the role of oxidative stress in patients with burns and chronic osteomyelitis, and demonstrate some specific features leading to formation of disease pathology. Such features of oxidative stress may be useful in future design of new approaches to correct the pathology of diseases.
Highlights
The purpose of this study was to evaluate and compare the features of the initiation and development of oxidative stress in patients with osteomyelitis and burns
It should be noted that both burns and chronic osteomyelitis show a decrease in antioxidant potential of the plasma
Analysis of the level of malonic dialdehyde in blood plasma and red blood cells allowed us to verify the specificity of the implementation of oxidative stress in burns and chronic osteomyelitis (Figure 3 and Figure 4)
Summary
The purpose of this study was to evaluate and compare the features of the initiation and development of oxidative stress in patients with osteomyelitis and burns. In burn victims, oxidative stress signs had a maladaptive character They were seen in blood plasma and red blood cells, and accompanied by a pronounced depletion of enzyme antioxidant system reserves. It is known that the reactions of free radicals and the opposing activity of a wide range of antioxidant systems are the fundamental basis for various aspects of the functioning of cells and tissues [1,2,3,4] They contribute to the renewal of cellular structures and processes, including biological membranes, energy metabolism, and phagocytic reactions [1,4,5,6,7]. Signs of oxidative stress include a pronounced activation of free radical processes, accompanied by a significant inhibition of the corresponding blood and tissue systems [5,13,15,16,17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
