The influence of agar brands and micronutrients in the growth optimization of Granulicella sp. (Acidobacteriota)

Ohana Y.A Costa,Eiko E Kuramae

doi:10.1016/j.mimet.2021.106148

Ohana Y.A Costa, Eiko E Kuramae

Open Access

https://doi.org/10.1016/j.mimet.2021.106148

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Abstract

Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should be carefully considered for Acidobacteriota strains growth and microbial isolation.

Highlights

One of the major hurdles of studying bacteria of phylum Acid obacteriota is their isolation and propagation in culture media
Our results showed that Granulicella strain 5B5 was not able to grow on Sigma Agar A1296-500, which poorly supported the growth of Granulicella strain WH15
The metal ion composition of the different agar brands has been previously addressed by Bosmans et al (Bosmans et al, 2016)

Summary

Introduction

One of the major hurdles of studying bacteria of phylum Acid obacteriota is their isolation and propagation in culture media. Our results showed that Granulicella strain 5B5 was not able to grow on Sigma Agar A1296-500, which poorly supported the growth of Granulicella strain WH15. Fluka Agar 05038–500 supported the growth of both strains, but results demonstrated the Granulicella strains per formed the best in BD agar (Fig. 1), and was not temperature-dependent.

Results

Conclusion