Abstract
Objective To investigate the influence of adipose mesenchymal stem cell (AMSC) on immune status and transplantation effect when co-transplanted with allogenic islets. Methods The streptozotocin induced diabetic Lewis rats were selected as islet transplantation recipients. The diabetic rats which were co-transplanted with islets from Wistar rats and AMSC from Lewis rats were set as the AMSC+ islet transplantation group. The rats in islet transplantation group were transplanted with islets from Wistar rats. The rats in AMSC transplantation group were transplanted with AMSC from Lewis rats. All the islets and AMSC were transplanted through subrenal capsular of left kidney. The rats in positive control group were diabetic Lewis rats. The rats in negtive control group were normal Lewis rats. The concentrations of IFN-γ, IL-2, IL-4 and IL-10 in peripheraI blood serum was detected with ELISA, the average percentage of CD4+ T and CD8+ T cells in peripheral blood was tested with flow cytometry, the infiltration of lymphocytes in the islets grafts was observed by HE staining. Blood glucose and serum insulin levels of the rats and survival time of the islet grafts were observed. Results The mean survival time of the islet grafts was longer in AMSC+ islet transplantation group [(26.8±3.0) days] than in islet transplantation group [(19.0±1.3) days], and statistical differences was also found for survival curve of islets grafts between the two groups (χ2=4.494, P 0.05). On the 7th, 14th and 28th days after transplantation, the serum concentrations of IFN-γ and IL-2 in the AMSC+ islet transplantation group were significantly lower than those in the islet transplantation group (P all <0.05), and the serum concentrations of IL-4 and IL-10 significantly higher than those in the islet transplantation group (P all <0.05), the average percentage of peripheral blood CD4+ T and CD8+ T cells less than those in the islet transplantation group (P all <0.05). On the 7th day after transplantation, the infiltrating lymphocyte counts of islet grafts in the AMSC+ islet transplantation group were significantly lower than those in the islet transplantation group (t=8.245, P<0.05). Conclusions In diabetic rats, AMSC could improve the effects of islets transplantation, regulate the expression of IFN-γ, IL-2, IL-4 and IL-10, suppress the T lymphocyte proliferation, and reduce islet grafts immune injury. Key words: Adipose mesenchymal stem cell; Islet transplantation; Rejection; Diabetes mellitus; Co-transplant; Rats
Published Version
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