THE INFLUENCE OF 3-(6,11-DIOXO-6,11-DIHYDRO-12H-BENZO[b]PHENOXAZIN-12-YL)BUTANOIC ACID ON RAPESEED MORPHOGENESIS IN VITRO AND GLUCOSINOLATE AND THIOCYANATE QUANTITIES

Abstract

Naphthoquinones are widely distributed in plants, fungi, and some animals. Their biological activities have been studied including their effects on prokaryotic and eukaryotic cells. Vitamin K is a derivative of 1,4-naphthoquinone. Other natural naphtoquinones include juglone, plumbagin, droserone. Naphthoquinone derivatives have significant pharmacological properties: they are cytotoxic, have a significant antibacterial, antifungal, antiviral, insecticidal, anti-inflammatory and antipyretic properties. Menadione sodium bisulphite (MSB) increased the growth of tomato plants and alfalfa callus and stimulated rooting of mungbean cuttings. The levels of IAA were increased by about 3 to 4 fold following application of MSB to tomato, cucumber, corn and capsicum plants. Plumbagone, juglone and lawsone are naturally occurring naphthoquinones of plant origin that have antibacterial effects on several species of both aerobic and anaerobic organisms, and toxins derived from naphthazarin (5,8-dihydroxy-1,4-naphthoquinone) are produced by Fusarium solani and attack plants, other fungi and bacteria. Oilseed rape, Brassica napus L., is one of the most important crops for production of vegetable oils in the world. Traditional rapeseed cultivars contain high amounts of glucosinolates. Although glucosinolates have antibacterial, antifungal properties and cancerchemoprevention activity, their antinutritional effects have limited the use of meals from oilseed rape for human food and animal feed. Besides, these compounds provide the bitter flavour and sulfurous aroma due to breakdown. Therefore, one of the main biotechnology objective is reduction of glucosinolates content in seeds to trace amounts. Rapeseed and its products are rich sources of bioactive compounds such as: polyphenols, phytosterols, tocopherols and other antioxidants, which are important in the prevention and treatment of coronary heart diseases, cancers, diabetes, hypertension, neurodegenerative and autoimmune diseases. Plant growth regulators are the critical media components in determining the developmental pathway of the plant cells. The regeneration of plants in vitro is interesting not only for studies of the regeneration process itself but also for success of in vitro manipulations of crop improvement. The main object was to explore the influence of 3-(6,11-dioxo-6,11-dihydro-12H-benzo[b]phenoxazin-12-yl)butanoic acid on rapeseed morphogenesis in vitro and glucosinolate and thiocyanate quantities. 3-(6,11-Dioxo-6,11-dihydro-12H-benzo[b]phenoxazin-12-yl)butanoic acid was synthesized at the Department of Organic Chemistry. We used Murashige-Skoog medium with 6-benzylaminopurine (BAP) (2 mg/l) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.2 mg/l) for obtaining rapeseed callus cultures in vitro. Compounds were examined at different concentrations 0.5 mg/l; 1.0 mg/l; 1.5 mg/l; 2.0 mg/l in the medium. It was determined, that the growth of rapeseed „Sponsor“ sprouts stimulated and biomass increased of rapeseed, which were grown on MS medium with 3-(6,11-dioxo-6,11-dihydro-12H-benzo[b]phenoxazin-12-yl)butanoic acid (1 mg/l). It was explored, that the lowest amount of glucosinolates was in rapeseed overground parts, which were grown on MS medium with 3-(6,11-dioxo-6,11-dihydro-12H-benzo[b]phenoxazin-12-yl)butanoic acid (2 mg/l) and the lowest amount of thiocyanates was in rapeseed roots, which were grown on MS medium with 3-(6,11-dioxo-6,11-dihydro-12H-benzo[b]phenoxazin-12-yl)butanoic acid (1.5 mg/l).DOI: http://dx.doi.org/10.5755/j01.ct.60.2.1948