THE INFLUENCE OF 2-OXOINDOLINE-3-GLYOXYLIC ACID DERIVATIVES ON THE NEUROPHYSIOLOGICAL REACTIONS OF RATS IN THE “BLACK AND WHITE CHAMBER” TEST

Abstract

The number of patients with an anxiety pathology is constantly growing, which requires the search and development of new remedies for protection. The aim of this research is to establish the presence of anxiolytic activity in derivatives of 2-oxoindolin-3-glyoxylic acid basedon testing animals in the “black-and-white chamber” test. Materials and methods. Experiments were performed on 150 adult male rats. The 2-oxoindoline derivatives were suspended ex tempore (water for injection and Tween-80) and administered to rats at a dose of 12 mg/kg intraperitoneally 1 hour before testing. The control group received a solvent with an emulsifier. Diazepam (2 mg/kg) served as a reference preparation. Behavior was studied in the “black and white chamber” test. Results. Compound 2 significantly reduced the residence time in the light compartment of the chamber. Substance 18 reduced the latent period of the first peek by 1.4 times (p<0.01), significantly increased the number and time of peeking, reduced the latent period of exit by 1.6 times (p<0.001), increased the number of exits and the time spent in the illuminated part of the device and significantly increased the percentage of rats peeking or exiting from the dark compartment and also reduced the number of boluses by 1.9-fold (p<0.001) compared to the control. Substance 2-T only increased the total time of peeking, compound K significantly reduced the number of boluses, and compound 3.85 increased the latent period of the first peek by 1.5 times (p<0.002), substance G increased the number of peeks by 1.7 times ( p<0.05), the time of stay in the light compartment of the camera by 2.5 times (p<0.001) and reduced the number of boluses by 1.3 times compared to the injection control (p<0.05). Conclusion. Administration of the IK compound significantly prolonged the latency of the first peek and exit, as well as significantly reduced the number of boluses compared to the control. 100% of animals peeked into the illuminated compartment of the chamber. Derivatives of 2-oxoindoline with the laboratory codes 18, G, and IК changed the neurophysiological reactions of animals in a similar way to the classic anxiolytic diazepam.