The inflammatory response to implanted materials, fibrous capsule characterisation

Abstract

Event Abstract Back to Event The inflammatory response to implanted materials, fibrous capsule characterisation Brooke Farrugia1, John Whitelcok1 and Megan Lord1 1 UNSW Australia, Graduate School of Biomedical Engineering, Australia A major problem associated with the implantation of foreign materials is their propensity to induce inflammation and fibrosis. While the infiltration of inflammatory cells and formation of a fibrous capsule around the implanted material are relatively well characterised, research into the mechanistic events that occur are still relatively unknown. Recruitment of mast cells, to sites of material implantation is thought to be involved in the recruitment of inflammatory cells, through the release of pro-inflammatory proteases and cytokines on activation from their α-granules. This study aims to investigate the role that mast cells have on the inflammatory response to implanted materials and fibrous capsule formation. Test materials for implantation consisted of the naturally occurring polymer Chitosan, with and without acid pre-treatment, and Surgicel, a commercially available cellulose haemostatic agent. Materials were subcutaneously implanted into rats for 7 days and following explanation histological characterisation was carried out through H&E, picrosirrus red and Leder stain, as well as immunohistochemistry. Results showed the inflammatory response varied between the different material implants, as indicated by the thickness of the fibrous capsule and distribution of collagen. Presence and location of Leder stain positive cells were shown within the fibrous capsule in response to all material implants. The mast cell receptor, c-kit, as well as proteoglycans (PGs) and glycosaminoglycan (GAGs) known to be produced by mast cells, serglycin, perlecan and chondroitin sulphate, were shown to be expressed with the fibrous capsule, with differing expression and distribution, as well as co-localisation with other markers. These data support the role that mast cells play in the inflammatory host response to material implants, where mediators released from their α-granules impact on the formation of a collagen dense fibrous capsule. Highlighting the importance of ECM characterisation in addition to collagen, and the potential use of PGs as inflammatory markers. Dr Simon McCarthy; Dr Barbara McGrath Keywords: biomaterial, Implant Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in immune response Citation: Farrugia B, Whitelcok J and Lord M (2016). The inflammatory response to implanted materials, fibrous capsule characterisation. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00994 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Brooke Farrugia John Whitelcok Megan Lord Google Brooke Farrugia John Whitelcok Megan Lord Google Scholar Brooke Farrugia John Whitelcok Megan Lord PubMed Brooke Farrugia John Whitelcok Megan Lord Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.